Aseptic Technique and Culturing Microbes

Microbial identification experiments:

• Biochemical Testing for Microbial Identification—Methyl Red, Voges-Proskauer, and Catalase LabPaq experiment using Escherichia coli and Staphylococcus epidermidis

• Biochemical Testing for Microbial Identification—Carbohydrate Fermentation LabPaq experiment using Saccharomyces cerevisiae and Staphylococcus epidermidis

• Bacterial Identification through Functional Media—Motility Testing LabPaq experiment using Escherichia coli and Staphylococcus epidermidis

Note: You will need the Staphylococcus epidermidis culture for Task 3, so do not discard it.

Your lab report should be written in complete sentences and in paragraph form. Your report should include the following sections:

• introduction

• materials and methods

• results

• discussion

The information required for each section is outlined in the requirements section below.

Requirements:

Your submission must be your original work. No more than a combined total of 30% of the submission and no more than a 10% match to any one individual source can be directly quoted or closely paraphrased from sources, even if cited correctly. Use the Turnitin Originality Report available in Taskstream as a guide for this measure of originality.

You must use the rubric to direct the creation of your submission because it provides detailed criteria that will be used to evaluate your work. Each requirement below may be evaluated by more than one rubric aspect. The rubric aspect titles may contain hyperlinks to relevant portions of the course.

A. Write a lab report about the Aseptic Technique and Culturing Microbes LabPaq experiment and the microbial identification experiments in which you do the following:

1. Discuss the importance of using proper aseptic technique when working with microorganisms.

Note: The discussion from part A1 belongs in the introduction section of the lab report.

2. Describe how the following experiments were performed, including the organisms used, media used, the time of incubation, and any reagents that were added:

a. Biochemical Testing for Microbial Identification—Methyl Red, Voges-Proskauer, and Catalase LabPaq experiment

b. Biochemical Testing for Microbial Identification—Carbohydrate Fermentation LabPaq experiment

c. Bacterial Identification through Functional Media—Motility Testing LabPaq experiment

Note: The descriptions from parts A2a–c belong in the materials and methods section of the lab report.

3. Record your observed results for each microbial identification experiment performed on the following organisms:

a. Escherichia coli

b. Staphylococcus epidermidis

c. Saccharomyces cerevisiae

Note: The observed results from part A3a–c belong in the results section of the lab report. You may use the attached “Data Tables” to record your results to help you organize your response.

4. Interpret your observed results by explaining why the following organisms reacted the way they did in each microbial identification experiment performed:

a. Escherichia coli

b. Staphylococcus epidermidis

c. Saccharomyces cerevisiae

Note: The descriptions from part A4a–c belong in the discussion section of the lab report.

Note: Only provide interpretations for the results obtained for the tests performed on each organism. For example, Escherichia coli was not tested for carbohydrate fermentation, so a discussion of the carbohydrate fermentation of Escherichia coli would not be included.

B. Acknowledge sources, using in-text citations and references, for content that is quoted, paraphrased, or summarized.

C. Demonstrate professional communication in the content and presentation of your submission.